4 Mar
2014
4 Mar
'14
7:56 a.m.
Hello trinity users I used Trinity to assemble ~700M paired-end reads using the following command "Trinity.pl --seqType fq --left left.fq --right right.fq --SS_lib_type FR --output ./ --JM 300G --CPU 15" The run was successful, and I got a list of contigs. Now I realize that the data was not strand specific, and I am wondering how does the adding of "--SS_lib_type FR" parameter affect the assembly. Shall I redo the assembly? Thanks Mali