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tde-users March 2014

users@trinitydesktop.org

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SS_lib_type question
by mali salmon 04 Mar '14

04 Mar '14

Hello trinity users I used Trinity to assemble ~700M paired-end reads using the following command "Trinity.pl --seqType fq --left left.fq --right right.fq --SS_lib_type FR --output ./ --JM 300G --CPU 15" The run was successful, and I got a list of contigs. Now I realize that the data was not strand specific, and I am wondering how does the adding of "--SS_lib_type FR" parameter affect the assembly. Shall I redo the assembly? Thanks Mali

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ExeGnu asks for User + Password
by jotatb 01 Mar '14

01 Mar '14

Hi, I just installed ExeGnu on a USB stick, but KDM asks for user and password. I tried everything I could imagine, but nothing worked. Can you help, please? Thanks Jose Tadeu

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